![]() Acta Neuropathol Commun 7:16ĬDC (2019) Answering questions about chronic traumatic encephalopathy (CTE). All immunohistochemistry performed on 4 μm sections from standard-sized blocks of formalin-fixed (10% neutral buffered formalin), paraffin-embedded tissue on a Leica BOND-MAX™ autostainer using the Leica BOND Polymer Refine detection system as per the manufacturer’s recommendationsīuckland ME, Sy J, Szentmariay I, Kullen A, Lee M, Harding A, Halliday G, Suter CM (2019) Chronic traumatic encephalopathy in two former Australian National Rugby League players. The boxed area is represented at high power in the inset. g Beta-amyloid (betaA4 clone 6F/3D, 1:50 dilution) immunoreactivity in superior frontal cortex (Brodmann area 8). f pTDP-43 (clone 1D3, 1:500 dilution) staining of temporal lobe in the same superficial cortical layers depicted in ( c), showing positive neuronal cytoplasmic inclusions and short neurites. There was accompanying moderate neuronal loss, pigment incontinence and gliosis. ![]() e Widespread pTau staining (as both globose tangles and pretangle pathology) in neurons of the substantia nigra, with accompanying neuritic pathology. d Inferior temporal gyrus from another individual (77yo ex-ARF player with AD but no CTE), showing a pattern of pTau pathology distinct to that of CTE, with neuronal pTau staining concentrated in deeper cortical layers and dense neuritic staining. pTau is also present in deeper cortical layers as irregular/patchy clumps of mixed neuronal and astrocytic staining. This superficial pTau is more evenly distributed throughout temporal cortex, with only occasional denser foci at sulcal depths (four foci across four blocks of anterior temporal lobe). c pTau staining of anterior superior temporal lobe (Brodmann area 38), showing dense immunoreactivity of both neurons and astrocytes concentrated in superficial cortical layers (layers II-III). The boxed area in ( a) is represented at high power in ( b). pTau is found in the soma and processes of both neurons and astrocytes in an irregular distribution concentrated around blood vessels: the defining lesion of CTE. a, b pTau (clone AT8, 1:800 dilution) immunoreactivity concentrated at the depths of a cortical sulcus in the superior frontal cortex (Brodmann area 8). Neuronal pTau was composed of both 3R and 4R isoforms, while astrocytic pTau was predominantly 4R. This pattern of pTau deposition, commonly seen in severe CTE, is distinct from the typical pTau deposition in AD (Fig. 1c), consisting of pretangle and tangle pTau, and some ghost tangles. In the temporal and insular cortices there was also dense involvement of superficial layers (layers II-III) (Fig. Twelve CTE foci were present within nine frontal lobe blocks, and four foci in four temporal lobe blocks. Neocortical pTau was markedly concentrated in an irregular perivascular distribution at sulcal depths in the soma and processes of both neurons and astrocytes: this is the defining lesion of CTE (Fig. Phosphorylated Tau immunoreactivity (pTau) was present in many grey matter regions. There was mild-moderate frontal and temporal lobe atrophy with ex-vacuo ventriculomegaly (lateral and third ventricles), mild uncomplicated atheroma in the basal vasculature, and pallor of the substantia nigra. Table 1 summarises the relevant neuropathology.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |